Lambda DNA (dam–dcm–)

Description
Lambda DNA (dam-, dcm-) is commonly used as a substrate for restriction enzymes sensitive to Dam or Dcm methylation. The phage is isolated from a heat-inducible lysogenic E.coli GM2163 (cI857 Sam7) strain. The DNA is isolated from purified phage by standard purification procedure.
The molecule is linear a double-stranded DNA, 48502 base pairs in length.
For detailed information see.

Molecular Weight
31.5 x 10⁶ daltons.

Feature
Used as a substrate for restriction enzymes sensitive to Dam or Dcm methylation.

Concentration
0.3 µg/µl.

Storage Buffer
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.

Quality Control
Gel analysis for purity and homogeneity of BclI, Eco31I, TaqI and XbaI fragmentation patterns